Microscopy Facility   
Text Karl-Franzens-Universitaet logo uni graz
    Microscopy Home  |   YGMBG Graz Home  |   IMB Graz Home
About us   
Downloads I*   
Downloads II*   
Teaching material*   


*Authorization required

Heimo Wolinski, PhD
University Graz
Senior Scientist
Humboldtstrasse 50/II
8010 Graz, Austria
Phone: +43 316 380 5489
Fax: +43 316 380 9854

Publications & Talks



Spribille T, Tuovinen V, Resl P, Vanderpool D, Wolinski H, Aime MC, Schneider K, Stabentheiner E, Toome-Heller M, Thor G, Mayrhofer H, Johannesson H, McCutcheon JP. Basidiomycete yeasts in the cortex of ascomycete macrolichens. 2016. Science. 29;353(6298):488-92.

Grond, Susanne; Radner, Franz P. W.; Eichmann, Thomas O.; Kolb, Dagmar; Grabner, Gernot F.; Wolinski, Heimo; Gruber, Robert; Hofer, Peter; Heier, Christoph; Schauer, Silvia; Rulicke, Thomas; Hoefler, Gerald; Schmuth, Matthias; Elias, Peter M.; Lass, Achim; Zechner, Rudolf; Haemmerle, Guenter. 2016. Skin Barrier Development Depends on CGI-58 Protein Expression During Late-Stage Keratinocyte Differentiation. The Journal of investigative dermatology. 10.1016/j.jid.2016.09.025

Nusshold C, Üllen A, Kogelnik N, Bernhart E, Reicher H, Plastira I, Glasnov T, Zangger K, Rechberger G, Kollroser M, Fauler G, Wolinski H, Weksler BB, Romero IA, Kohlwein SD, Couraud PO, Malle E, Sattler W. Assessment of electrophile damage in a human brain endothelial cell line utilizing a clickable alkyne analog of 2-chlorohexadecanal.Free Radic Biol Med. 2016 Jan;90:59-74. doi: 10.1016/j.freeradbiomed.2015.11.010.

Heier C, Taschler U, Radulovic M, Aschauer P, Eichmann TO, Grond S, Wolinski H, Oberer M, Zechner R, Kohlwein SD, Zimmermann R. Monoacylglycerol lipases act as evolutionarily conserved regulators of non-oxidative ethanol metabolism. J Biol Chem. 2016 Mar 31. pii:jbc.M115.705541. [Epub ahead of print]


H. Wolinski* (*corresponding author), H.F. Hofbauer, K. Hellauer, A. Cristobal-Sarramian, D. Kolb, M. Radulovic, O.L. Knittelfelder, G.N. Rechberger, S.D. Kohlwein, Seipin is involved in the regulation of phosphatidic acid metabolism at a subdomain of the nuclear envelope in yeast, Biochimica et biophysica acta (2015). 10.1016/j.bbalip.2015.08.003.

Monoglyceride lipase deficiency causes desensitization of intestinal cannabinoid receptor type 1 and increased colonic ?-opioid receptor sensitivity (Article)
Taschler, U.a, Eichmann, T.O.a, Radner, F.P.W.a, Grabner, G.F.a, Wolinski, H.a,
Storr, M.b, Lass, A.a, Schicho, R.c, Zimmermann. 2015. British Journal of Pharmacology Volume 172, Issue 17, Pages 4419-4429

Pribasnig MA, Mrak I, Grabner GF, Taschler U, Knittelfelder O, Scherz B, Eichmann TO, Heier C, Grumet L, Kowaliuk J, Romauch M, Holler S, Anderl F, Wolinski H, Lass A, Breinbauer R, Marsche G, Brown JM, Zimmermann R. ?/? Hydrolase Domain-containing 6 (ABHD6) Degrades the Late Endosomal/Lysosomal Lipid Bis(monoacylglycero)phosphate. J Biol Chem. 2015 Dec 11;290(50):29869-81. doi: 10.1074/jbc.M115.669168

Taschler, U., Schreiber, R., Chitraju, C., Grabner, G.F., Romauch, M., Wolinski, H., Haemmerle, G., Breinbauer, R., Zechner, R., Lass, A., Zimmermann, R. Adipose triglyceride lipase is involved in the mobilization of triglyceride and retinoid stores of hepatic stellate cells (2015) Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1851 (7), pp. 937-945.

Niso-Santano, M., Malik, S.A., Pietrocola, F., Bravo-San Pedro, J.M., Mariño, G., Cianfanelli, V., Ben-Younès, A., Troncoso, R., Markaki, M., Sica, V., Izzo, V., Chaba, K., Bauvy, C., Dupont, N., Kepp, O., Rockenfeller, P., Wolinski, H., Madeo, F., Lavandero, S., Codogno, P., Harpur, F., Pierron, G., Tavernarakis, N., Cecconi, F., Maiuri, M.C., Galluzzi, L., Kroemur, G. Unsaturated fatty acids induce non-canonical autophagy (2015) EMBO Journal, 34 (8), pp. 1025-1041.

Kuijper (corr. author), A.a , Heise, B.b, Zhou, Y.c, He, L.d, Wolinski, H.e,
Kohlwein. (2015). Segmentation of clustered cells in microscopy images by geometric pdes and level sets. Handbook of Biomedical Imaging: Methodologies and Clinical Research, p 475-487

Wolinski, H., Kohlwein, S.D. Microscopic and spectroscopic techniques to investigate lipid droplet formation and turnover in yeast (2015) Methods in Molecular Biology, 1270, pp. 289-305.


Mattiazzi Ušaj, M., Brložnik, M., Kaferle, P., Žitnik, M., Wolinski, H., Leitner, F., Kohlwein, S.D., Zupan, B., Petrovic, U.Genome-Wide Localization Study of Yeast Pex11 Identifies Peroxisome-Mitochondria Interactions through the ERMES Complex (2014) Journal of Molecular Biology, Article in Press.

Hofbauer, H.F., Schopf, F.H., Schleifer, H., Knittelfelder, O.L., Pieber, B., Rechberger, G.N., Wolinski, H., Gaspar, M.L., Kappe, C.O., Stadlmann, J., Mechtler, K., Zenz, A., Lohner, K., Tehlivets, O., Henry, S.A., Kohlwein, S.D. Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids (2014) Developmental Cell, 29 (6), pp. 729-739.

Van Zutphen, T., Todde, V., De Boer, R., Kreim, M., Hofbauer, H.F., Wolinski, H., Veenhuis, M., Van Der Klei, I.J., Kohlwein, S.D. Lipid droplet autophagy in the yeast Saccharomyces cerevisiae (2014) Molecular Biology of the Cell, 25 (2), pp. 290-301.

Wolinski, H., Kohlwein, S.D. Single yeast cell imaging (2014) Methods in Molecular Biology, 1205, pp. 91-109.


Schernthaner, M., Leitinger, G., Wolinski, H., Kohlwein, S.D., Reisinger, B., Barb, R.-A., Graier, W.F., Heitz, J., Groschner, K. Enhanced Ca2+ entry and tyrosine phosphorylation mediate nanostructure-induced endothelial proliferation (2013) Journal of Nanomaterials, 2013, art. no. 251063,

Sommer G, Schwarz M, Kutschera M, Kresnik R, Regitnig P, Schriefl AJ, Wolinski H, Kohlwein SD, Holzapfel GA. Biomechanical Properties of the Human Ventricular Myocardium. (2014) Biomed Tech (Berl). 2013 Sep 7. [Epub ahead of print]

Radulovic, M., Knittelfelder, O., Cristobal-Sarramian, A., Kolb, D., Wolinski, H., Kohlwein, S.D. The emergence of lipid droplets in yeast: Current status and experimental approaches (2013) Current Genetics, 59 (4), pp. 231-242.

Chitraju, C., Trötzmüller, M., Hartler, J., Wolinski, H., Thallinger, G.G., Haemmerle, G., Zechner, R., Zimmermann, R., Köfeler, H.C., Spener, F. The impact of genetic stress by ATGL deficiency on the lipidome of lipid droplets from murine hepatocytes (2013) Journal of Lipid Research, 54 (8), pp. 2185-2194.

Aflaki, E., Balenga, N.A.B., Luschnig-Schratl, P., Wolinski, H., Povoden, S., Chandak, P.G., Bogner-Strauss, J.G., Eder, S., Konya, V., Kohlwein, S.-D., Heinemann, A., Kratky, D.

Schriefl, A.J., Wolinski, H., Regitnig, P., Kohlwein, S.D., Holzapfel, G.A. An automated approach for three-dimensional quantification of fibrillar structures in optically cleared soft biological tissues. (2013) Journal of the Royal Society, Interface / the Royal Society, 10 (80), p. 20120760.


Chitraju, C., Trötzmüller, M., Hartler, J., Wolinski, H., Thallinger, G.G., Lass, A., Zechner, R., Zimmermann, R., Köfeler, H.C., Spener, F. Lipidomic analysis of lipid droplets from murine hepatocytes reveals distinct signatures for nutritional stress (2012) Journal of Lipid Research, 53 (10), pp. 2141-2152.

Schernthaner, M., Reisinger, B., Wolinski, H., Kohlwein, S.D., Trantina-Yates, A., Fahrner, M., Romanin, C., Itani, H., Stifter, D., Leitinger, G., Groschner, K., Heitz, J. Nanopatterned polymer substrates promote endothelial proliferation by initiation of ?-catenin transcriptional signaling (2012) Acta Biomaterialia, 8 (8), pp. 2953-2962.

Mattiazzi, M., Sun, Y., Wolinski, H., Bavdek, A., Petan, T., Anderluh, G., Kohlwein, S.D., Drubin, D.G., Križaj, I., Petrovi?, U. A neurotoxic phospholipase A2 impairs yeast amphiphysin activity and reduces endocytosis (2012) PLoS ONE, 7 (7), art. no. e40931,

Paar, M., Jüngst, C., Steiner, N.A., Magnes, C., Sinner, F., Kolb, D., Lass, A., Zimmermann, R., Zumbusch, A., Kohlwein, S.D., and Wolinski, H. Remodeling of lipid droplets during lipolysis and growth in adipocytes (2012) Journal of Biological Chemistry, 287 (14), pp. 11164-11173.

Reisner, A., Wolinski, H., Zechner, E.L. In situ monitoring of IncF plasmid transfer on semi-solid agar surfaces reveals a limited invasion of plasmids in recipient colonies (2012) Plasmid, 67 (2), pp. 155-161.

Wolinski, H., Bredies, K., Kohlwein, S.D. Quantitative Imaging of Lipid Metabolism in Yeast: From 4D Analysis to High Content Screens of Mutant Libraries (2012) Methods in Cell Biology, 108, pp. 345-365.


Aflaki, E., Balenga, N.A.B., Luschnig-Schratl, P., Wolinski, H., Povoden, S., Chandak, P.G., Bogner-Strauss, J.G., Eder, S., Konya, V., Kohlwein, S.-D., Heinemann, A., Kratky, D. Impaired Rho GTPase activation abrogates cell polarization and migration in macrophages with defective lipolysis (2011) Cellular and Molecular Life Sciences, 68 (23), pp. 3933-3947. Cited 12 times.

Wolinski, H., Kolb, D., Hermann, S., Koning, R.I., Kohlwein, S.D. A role for seipin in lipid droplet dynamics and inheritance in yeast (2011) Journal of Cell Science, 124 (22), pp. 3894-3904.

Seper, A., Fengler, V.H.I., Roier, S., Wolinski, H., Kohlwein, S.D., Bishop, A.L., Camilli, A., Reidl, J., Schild, S. Extracellular nucleases and extracellular DNA play important roles in Vibrio cholerae biofilm formation (2011) Molecular Microbiology, 82 (4), pp. 1015-1037.

Bredies, K., Wolinski, H. An active-contour based algorithm for the automated segmentation of dense yeast populations on transmission microscopy images
(2011) Computing and Visualization in Science, 14 (7), pp. 341-352.

Aflaki, E., Radovi?, B., Chandak, P.G., Kolb, D., Eisenberg, T., Ring, J., Fertschai, I., Uellen, A., Wolinski, H., Kohlwein, S.-D., Zechner, R., Levak-Frank, S., Sattler, W., Graier, W.F., Malli, R., Madeo, F., Kratky, D. Triacylglycerol accumulation activates the mitochondrial apoptosis pathway in macrophages (2011) Journal of Biological Chemistry, 286 (9), pp. 7418-7428.

Kienesberger, S., Gorkiewicz, G., Wolinski, H., Zechner, E.L. New molecular microbiology approaches in the study of Campylobacter fetus (2011) Microbial Biotechnology, 4 (1), pp. 8-19.


Coassin, S., Schweiger, M., Kloss-Brandstätter, A., Lamina, C., Haun, M., Erhart, G., Paulweber, B., Rahman, Y., Olpin, S., Wolinski, H., Cornaciu, I., Zechner, R., Zimmermann, R., Kronenberg, F. Investigation and functional characterization of rare genetic variants in the adipose triglyceride lipase in a large healthy working population(2010) PLoS Genetics, 6 (12), art. no. e1001239, pp. 1-13.

Heier, C., Taschler, U., Rengachari, S., Oberer, M., Wolinski, H., Natter, K., Kohlwein, S.D., Leber, R., Zimmermann, R. Identification of Yju3p as functional orthologue of mammalian monoglyceride lipase in the yeast Saccharomyces cerevisiae (2010) Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1801 (9), pp. 1063-1071.

Gruber, A., Cornaciu, I., Lass, A., Schweiger, M., Poeschl, M., Eder, C., Kumari, M., Schoiswohl, G., Wolinski, H., Kohlwein, S.D., Zechner, R., Zimmermann, R., Oberer, M.
The N-terminal region of comparative gene identification-58 (CGI-58) is important for lipid droplet binding and activation of adipose triglyceride lipase (2010) Journal of Biological Chemistry, 285 (16), pp. 12289-12298.


Schreiber, R., Taschler, U., Wolinski, H., Seper, A., Tamegger, S.N., Graf, M., Kohlwein, S.D., Haemmerle, G., Zimmermann, R., Zechner, R., Lass, A. Esterase 22 and beta-glucuronidase hydrolyze retinoids in mouse liver (2009) Journal of Lipid Research, 50 (12), pp. 2514-2523.

Wolinski, H., Natter, K., Kohlwein, S.D. The fidgety yeast: Focus on high-resolution live yeast cell microscopy (2009) Methods in Molecular Biology, 548, pp. 75-99.

Petschnigg, J., Wolinski, H., Kolb, D., Zelling, G., Kurat, C.F., Natter, K., Kohlwein, S.D.
Good fat, essential cellular requirements for triacylglycerol synthesis to maintain membrane homeostasis in yeast (2009) Journal of Biological Chemistry, 284 (45), pp. 30981-30993

Beranek, A., Rechberger, G., Knauer, H., Wolinski, H., Kohlwein, S.D., Leber, R.
Identification of a cardiolipin-specific phospholipase encoded by the gene CLD1 (YGR110W) in yeast (2009) Journal of Biological Chemistry, 284 (17), pp. 11572-11578.

Kurat, C.F., Wolinski, H., Petschnigg, J., Kaluarachchi, S., Andrews, B., Natter, K., Kohlwein, S.D. Cdk1/Cdc28-Dependent Activation of the Major Triacylglycerol Lipase Tgl4 in Yeast Links Lipolysis to Cell-Cycle Progression (2009) Molecular Cell, 33 (1), pp. 53-63.

Wolinski, H., Petrovi?, U., Mattiazzi, M., Petschnlgg, J., Heise, B., Natter, K., Kohlwein, S.D. Imaging-based live cell yeast screen identifies novel factors involved in peroxisome assembly (2009) Journal of Proteome Research, 8 (1), pp. 20-27.


Wolinski, H., Kohlwein, S.D. Microscopic analysis of lipid droplet metabolism and dynamics in yeast (2008) Methods in Molecular Biology, 457, pp. 151-163.

Malanovic, N., Streith, I., Wolinski, H., Rechberger, G., Kohlwein, S.D., Tehlivets, O.
S-adenosyl-L-homocysteine hydrolase, key enzyme of methylation metabolism, regulates phosphatidylcholine synthesis and triacylglycerol homeostasis in yeast: Implications for homocysteine as a risk factor of atherosclerosis (2008) Journal of Biological Chemistry, 283 (35), pp. 23989-23999.

Kienesberger, P.C., Lass, A., Preiss-Landl, K., Wolinski, H., Kohlwein, S.D., Zimmermann, R., Zechner, R. Identification of an insulin-regulated lysophospholipase with homology to neuropathy target esterase (2008) Journal of Biological Chemistry, 283 (9), pp. 5908-5917.

- 2007

Eder, P., Probst, D., Rosker, C., Poteser, M., Wolinski, H., Kohlwein, S.D., Romanin, C., Groschner, K. Phospholipase C-dependent control of cardiac calcium homeostasis involves a TRPC3-NCX1 signaling complex (2007) Cardiovascular Research, 73 (1), pp. 111-119. Cited 46 times.

Kurat, C.F., Natter, K., Petschnigg, J., Wolinski, H., Scheuringer, K., Scholz, H., Zimmermann, R., Leber, R., Zechner, R., Kohlwein, S.D. Obese yeast: Triglyceride lipolysis is functionally conserved from mammals to yeast (2006) Journal of Biological Chemistry, 281 (1), pp. 491-500.

Natter, K., Leitner, P., Faschinger, A., Wolinski, H., McCraith, S., Fields, S., Kohlwein, S.D. The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy (2005) Molecular and Cellular Proteomics, 4 (5), pp. 662-672.

Wolinski, H. Confocal imaging reveals structural detail of the cell nucleus and ascospore formation in lichenized fungi (2003) Mycological Research, 107 (8), pp. 989-995.

Wolinski, H., Grube, M., Blanz, P. Direct PCR of symbiotic fungi using microslides
(1999) BioTechniques, 26 (3), pp. 454-455.

  • more....

  • footer bild
    UNI-NEWS    SITEMAP   SEARCH   UNI GRAZ         Webmaster | 28.06.2017