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Fluorescence microscopy courseMol 889 (VO, 2h) and MOl.890 (course)
Please note: the lecture exam (Mol.889) is required to attend the practical courses (Mol.890).
- A maximum number of 4 students per course is accepted.
- Written exam (Mol.889), active contribution, protocol and a summarizing oral presentation are expected.
Aim: make students acquainted with modern techniques in light and fluorescence microscopy in theory and practice; in depth discussion of modern techniques and their application.
Master + PhD students:
- Preparation techniques (yeast, bacteria, mammalian cells)
- Labeling techniques
- Live cell imaging and immunofluorescence
- Fluorescent proteins
- Bright field microscopy including contrast techniques such differential interference contrast (DIC),
- Hoffmanns modulation contrast (HMC).
- Wide-field fluorescence microscopy
- Confocal laser scanning microscopy (CLSM): determination of protein localization (2D, 3D), multi-labeling and spectral imaging, lambda scanning, spectral unmixing, protein dynamics (FRAP, FLIP)
- Image processing, 2D/3D visualization and image quantification techniques
(software: amira, Imaris, ImageJ, Photoshop CS4)
The course for the PhD students additionally includes:
- Application of 2-photon microscopy
- Studying protein interactions using FRET
- 4D live cell imaging (yeast and cell lines)
- Image deconvolution (Huygens)
- High speed imaging using resonant scanner
*** microscopes: Leica SP2 upright, Leica SP5 inverted***
+ BRING YOUR OWN SAMPLES!
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