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Four-dimensional live cell imagingFour-dimensional (4d) imaging (time-lapsed three-dimensional imaging) is a powerful tool for studying time-depended alternations of the subcellular localization and of morphological changes of spatial cellular structures. Tools for cultivation of yeast and mammalian cells directly on the microscope table and features provided by state-of-the art microscope systems for high-resolution imaging of multi-labelled specimens allow the detailed analysis of interactions of cellular structures in the three-dimensional context of the living cell.
4D live cell imaging and subsequent analysis of generated image data requires specific preparative conditions as well as specialized software tools. In this respect, our facility provides both hardware and software tools for efficient realization of 4D imaging experiments and for analysis of acquired complex 4D image data.
Lipid droplet inheritance during cellular growth in budding yeast. 4D series. Lipid droplets were labeled using BODIPY 493/503.
Heimo Wolinski (corresponding author) et al. 2015. Seipin is involved in the regulation of phosphatidic acid metabolism at a subdomain of the nuclear envelope in yeast. BBA. 1851(11):1450-64.
Margret Paar, Christian Juengst, Noemi A. Steiner, Christoph Magnes, Frank Sinner, Dagmar Kolb, Achim Lass, Robert Zimmermann, Andreas Zumbusch, Sepp D. Kohlwein and Heimo Wolinski. 2012. Remodeling of lipid droplets during lipolysis and growth in adipocytes. J. Biol. Chem. published February 6, 2012
Wolinski Heimo, Kolb Dagmar, Hermann Sandra, Koning Roman, Kohlwein Sepp-Dieter. 2011. A role for seipin in lipid droplet dynamics and inheritance in yeast. Journal of Cell Science. 124. p3894-3904.
Wolinski H, Natter K, Kohlwein SD (2009). The Fidgety Yeast: Focus on High-Resolution Live Yeast Cell Microscopy. Methods in Molecular Biology, Yeast Functional Genomics and Proteomics, vol. 548.
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