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Contact:
Heimo Wolinski, PhD
University Graz
Senior Scientist
Humboldtstrasse 50/II
8010 Graz, Austria
Phone: +43 316 380 5489
Fax: +43 316 380 9854
heimo.wolinski@uni-graz.at


Instrumentation

1. LEICA TCS SP5 AOBS CARS SHG 2-photon
  • Microscope: Leica DMI 6000 inverted microscope.
  • CARS based on PicoEMERALD solid-state-laser based light source (APE GmbH, HighQ Laser). Detection system for Second Harmonic Imaging.Two-photon microscopy (OPO). NDDs and noise-less HyD detectors.
  • Objectives: 100x 1.4 oil, 63x 1.4 oil, 63x 1.25 water, 40x 1.25 oil, 40x 1.2 air, 20x, 10x.
  • Differential interference contrast (DIC) optics.
  • Laser lines: Argon laser: 458nm/5mW, 476nm/5mW, 488nm/20mW, 496nm/5mW, 514nm/20mW; DPSS-laser: 561nm/20mW; HeNe-laser: 633nm/10mW.
  • Resonant scanner: 8000 Hz (25frames/sec 512x512).
  • DIC optics.
  • On stage incubation system (CO2, temperature, humidity control).


  • CARS info

  • CARS group Prof. Zumbusch/Konstanz

  • Leica SP5 info

  • A.P.E GmbH

  • HighQ Laser.




2. LEICA AOBS SP2 MP (confocal, two-photon)
  • Microscope: Leica DMRE upright microscope.
  • Objectives: HCX PL APO 100x/1.4-0.7 OIL CS; HCX PL APO 63x/1.32-0.6 OIL CS; HCX PL APO 40x/1.25-0.75 OIL CS; HC PL FLUOTAR 20x/0.5, HCX PL APO 63x/1.2 W CORR, HCX PL APO 63x/1.3 GLYC CORR CS, Leica 506505 10x.
  • Differential interference contrast (DIC) optics.
  • Laser lines: Ar/ArKr (458, 476, 488, 496, 514 nm), GreenHeNe (543 nm), HeNe (633 nm), 405nm diode laser, Spectra Physics MaiTai IR femto two-photon (780-920 nm) EOM.
  • Bioptechs objective heater system.


Leica SP2 AOBS MP





3. Leica TCS 4D (confocal)
  • Microscope: Leitz DMRBE upright microscope.
  • Objectives: PL APO 100x/1.4-0.7 OIL; PL APO 63x/1.4 OIL; PL FLUOTAR 40x/1 - 0.5 OIL; PL FLUOTAR 16x/0.5 IMM, PL FLUOTAR 10x/0.3.
  • Laser lines: Ar/ArKr (488, 543nm).
  • Video system: Hamamatsu Argus 10.
  • Differential interference contrast (DIC) optics.


Leica TCS 4D





TESLA image processing wokstation

  • INTEL Xeon E5 2.7 GHz 30 MB cache, GeForce GTX Titan 6MB, TESLA K40 12GB.




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