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Heimo Wolinski, PhD
University Graz
Senior Scientist
Humboldtstrasse 50/II
8010 Graz, Austria
Phone: +43 316 380 5489
Fax: +43 316 380 9854

Second harmonic generation (SHG) imaging

Nonlinear microscopy such as second harmonic generation has become an important tool for label-free imaging of biological samples. In SHG imaging laser light is focused on a sample to generate frequency doubled light. SHG light occurs at noncentrosymmetric loci which can be found at biological structures such as collagen, cellulose, myosin or specifically labeled cellular membranes.

We recently applied SHG for imaging of collagen structures of human aortas (~1.2 mm thickness).

Collagen structures of a human aorta imaged using SHG. Sample: Andreas Schriefl/Prof. Holzapfel, Institute of Biomechanics, TU-Graz.

Recent publications

Sommer G, Schriefl AJ, Andrä M, Sacherer M, Viertler C, Wolinski H, Holzapfel GA. Biomechanical properties and microstructure of human ventricular myocardium. Acta Biomater. 2015 Sep 15;24:172-92. doi: 10.1016/j.actbio.2015.06.031. Epub 2015 Jun 30.

Sommer G, Schwarz M, Kutschera M, Kresnik R, Regitnig P, Schriefl AJ, Wolinski H, Kohlwein SD, Holzapfel GA. 2013. Biomechanical Properties of the Human Ventricular Myocardium. Biomed Tech (Berl). 2013 Sep 7.

Schriefl Andreas, Wolinski Heimo, Reigitnig Peter, Kohlwein Sepp D., Holzapfel Gerhard A. 2013. An Automated Approach for 3D Quantification of Fibrillar Structures in Optically Cleared Soft Biological Tissues. Journal of the Royal Society Interface.10:20120760, 2013.

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